Understanding riparian vegetation condition across the Port Phillip and Westernport region is critical for guiding investment and managing emerging threats. The Streamside Vegetation Assessment (SVA) program, first implemented in 2021, was repeated in 2025 across 506 sites to support long-term monitoring of condition and trajectory. Results indicate that overall, vegetation condition has remained broadly stable, but with some localised differences between catchments and a significant increase in deer impacts, now affecting approximately one-third of sites. Increases in deer presence were particularly evident within the Yarra, Werribee and Maribyrnong catchments. The program has also evolved to support additional applications, including research on remote sensing and habitat suitability modelling. These findings highlight both the value of ongoing monitoring and the need for targeted management responses to emerging threats.